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utrack matlab analysis method  (MathWorks Inc)


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    MathWorks Inc utrack matlab analysis method
    ( A ) Representative phenotypes of EB3 localization in control and CKAP5 -silenced groups. NAR cells were transiently transfected with EB3.eGFP plasmid to label the microtubule + ends. After 12 hours of transfection, cells were further transfected with siControl/si CKAP5 LNPs, and live-cell imaging was performed after 30 hours of siRNA-mediated silencing. Live cell imaging was performed by a superresolution spinning disk microscope. Images were captured at 100×. Scale bars, 5 μm. Images were captured every second for a period of 90 s. The images represent superimposition images of 91 frames with each frame marked in a specific shade as shown in the time frame scale. Nondynamic + ends superimpose on each other in all 90 frames due to their static nature and represent as white due to superimposition, whereas dynamic + ends do not superimpose in all the 90 frames due to their dynamic behavior and thus appear as a rainbow color. Thus, higher dynamics of tubulin result in a wider range of colors in these representative superimposed images. ( B ) Quantitative representation of the microtubule (MT) growth speed in control and CKAP5 -silenced group. The live-cell kinetic data obtained from a spinning disk microscope was subjected to <t>Utrack</t> analysis using <t>MATLAB</t> software. ** P = 0.0097. ( C ) Quantitative representation of the MT growth lifetime in control- and CKAP5 -silenced group as measured by MATLAB analysis. ( D ) Quantitative representation of the MT growth length in control and CKAP5 -silenced group as measured by MATLAB analysis. For all the graphical analysis in (B) to (D), the data are represented as mean ± SEM from 10 mitotic events and statistically analyzed by an unpaired t test.
    Utrack Matlab Analysis Method, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/utrack matlab analysis method/product/MathWorks Inc
    Average 90 stars, based on 1 article reviews
    utrack matlab analysis method - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Therapeutic gene silencing of CKAP5 leads to lethality in genetically unstable cancer cells"

    Article Title: Therapeutic gene silencing of CKAP5 leads to lethality in genetically unstable cancer cells

    Journal: Science Advances

    doi: 10.1126/sciadv.ade4800

    ( A ) Representative phenotypes of EB3 localization in control and CKAP5 -silenced groups. NAR cells were transiently transfected with EB3.eGFP plasmid to label the microtubule + ends. After 12 hours of transfection, cells were further transfected with siControl/si CKAP5 LNPs, and live-cell imaging was performed after 30 hours of siRNA-mediated silencing. Live cell imaging was performed by a superresolution spinning disk microscope. Images were captured at 100×. Scale bars, 5 μm. Images were captured every second for a period of 90 s. The images represent superimposition images of 91 frames with each frame marked in a specific shade as shown in the time frame scale. Nondynamic + ends superimpose on each other in all 90 frames due to their static nature and represent as white due to superimposition, whereas dynamic + ends do not superimpose in all the 90 frames due to their dynamic behavior and thus appear as a rainbow color. Thus, higher dynamics of tubulin result in a wider range of colors in these representative superimposed images. ( B ) Quantitative representation of the microtubule (MT) growth speed in control and CKAP5 -silenced group. The live-cell kinetic data obtained from a spinning disk microscope was subjected to Utrack analysis using MATLAB software. ** P = 0.0097. ( C ) Quantitative representation of the MT growth lifetime in control- and CKAP5 -silenced group as measured by MATLAB analysis. ( D ) Quantitative representation of the MT growth length in control and CKAP5 -silenced group as measured by MATLAB analysis. For all the graphical analysis in (B) to (D), the data are represented as mean ± SEM from 10 mitotic events and statistically analyzed by an unpaired t test.
    Figure Legend Snippet: ( A ) Representative phenotypes of EB3 localization in control and CKAP5 -silenced groups. NAR cells were transiently transfected with EB3.eGFP plasmid to label the microtubule + ends. After 12 hours of transfection, cells were further transfected with siControl/si CKAP5 LNPs, and live-cell imaging was performed after 30 hours of siRNA-mediated silencing. Live cell imaging was performed by a superresolution spinning disk microscope. Images were captured at 100×. Scale bars, 5 μm. Images were captured every second for a period of 90 s. The images represent superimposition images of 91 frames with each frame marked in a specific shade as shown in the time frame scale. Nondynamic + ends superimpose on each other in all 90 frames due to their static nature and represent as white due to superimposition, whereas dynamic + ends do not superimpose in all the 90 frames due to their dynamic behavior and thus appear as a rainbow color. Thus, higher dynamics of tubulin result in a wider range of colors in these representative superimposed images. ( B ) Quantitative representation of the microtubule (MT) growth speed in control and CKAP5 -silenced group. The live-cell kinetic data obtained from a spinning disk microscope was subjected to Utrack analysis using MATLAB software. ** P = 0.0097. ( C ) Quantitative representation of the MT growth lifetime in control- and CKAP5 -silenced group as measured by MATLAB analysis. ( D ) Quantitative representation of the MT growth length in control and CKAP5 -silenced group as measured by MATLAB analysis. For all the graphical analysis in (B) to (D), the data are represented as mean ± SEM from 10 mitotic events and statistically analyzed by an unpaired t test.

    Techniques Used: Control, Transfection, Plasmid Preparation, Live Cell Imaging, Microscopy, Software



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    utrack matlab analysis method  (MathWorks Inc)
    90
    MathWorks Inc utrack matlab analysis method
    ( A ) Representative phenotypes of EB3 localization in control and CKAP5 -silenced groups. NAR cells were transiently transfected with EB3.eGFP plasmid to label the microtubule + ends. After 12 hours of transfection, cells were further transfected with siControl/si CKAP5 LNPs, and live-cell imaging was performed after 30 hours of siRNA-mediated silencing. Live cell imaging was performed by a superresolution spinning disk microscope. Images were captured at 100×. Scale bars, 5 μm. Images were captured every second for a period of 90 s. The images represent superimposition images of 91 frames with each frame marked in a specific shade as shown in the time frame scale. Nondynamic + ends superimpose on each other in all 90 frames due to their static nature and represent as white due to superimposition, whereas dynamic + ends do not superimpose in all the 90 frames due to their dynamic behavior and thus appear as a rainbow color. Thus, higher dynamics of tubulin result in a wider range of colors in these representative superimposed images. ( B ) Quantitative representation of the microtubule (MT) growth speed in control and CKAP5 -silenced group. The live-cell kinetic data obtained from a spinning disk microscope was subjected to <t>Utrack</t> analysis using <t>MATLAB</t> software. ** P = 0.0097. ( C ) Quantitative representation of the MT growth lifetime in control- and CKAP5 -silenced group as measured by MATLAB analysis. ( D ) Quantitative representation of the MT growth length in control and CKAP5 -silenced group as measured by MATLAB analysis. For all the graphical analysis in (B) to (D), the data are represented as mean ± SEM from 10 mitotic events and statistically analyzed by an unpaired t test.
    Utrack Matlab Analysis Method, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/utrack matlab analysis method/product/MathWorks Inc
    Average 90 stars, based on 1 article reviews
    utrack matlab analysis method - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) Representative phenotypes of EB3 localization in control and CKAP5 -silenced groups. NAR cells were transiently transfected with EB3.eGFP plasmid to label the microtubule + ends. After 12 hours of transfection, cells were further transfected with siControl/si CKAP5 LNPs, and live-cell imaging was performed after 30 hours of siRNA-mediated silencing. Live cell imaging was performed by a superresolution spinning disk microscope. Images were captured at 100×. Scale bars, 5 μm. Images were captured every second for a period of 90 s. The images represent superimposition images of 91 frames with each frame marked in a specific shade as shown in the time frame scale. Nondynamic + ends superimpose on each other in all 90 frames due to their static nature and represent as white due to superimposition, whereas dynamic + ends do not superimpose in all the 90 frames due to their dynamic behavior and thus appear as a rainbow color. Thus, higher dynamics of tubulin result in a wider range of colors in these representative superimposed images. ( B ) Quantitative representation of the microtubule (MT) growth speed in control and CKAP5 -silenced group. The live-cell kinetic data obtained from a spinning disk microscope was subjected to Utrack analysis using MATLAB software. ** P = 0.0097. ( C ) Quantitative representation of the MT growth lifetime in control- and CKAP5 -silenced group as measured by MATLAB analysis. ( D ) Quantitative representation of the MT growth length in control and CKAP5 -silenced group as measured by MATLAB analysis. For all the graphical analysis in (B) to (D), the data are represented as mean ± SEM from 10 mitotic events and statistically analyzed by an unpaired t test.

    Journal: Science Advances

    Article Title: Therapeutic gene silencing of CKAP5 leads to lethality in genetically unstable cancer cells

    doi: 10.1126/sciadv.ade4800

    Figure Lengend Snippet: ( A ) Representative phenotypes of EB3 localization in control and CKAP5 -silenced groups. NAR cells were transiently transfected with EB3.eGFP plasmid to label the microtubule + ends. After 12 hours of transfection, cells were further transfected with siControl/si CKAP5 LNPs, and live-cell imaging was performed after 30 hours of siRNA-mediated silencing. Live cell imaging was performed by a superresolution spinning disk microscope. Images were captured at 100×. Scale bars, 5 μm. Images were captured every second for a period of 90 s. The images represent superimposition images of 91 frames with each frame marked in a specific shade as shown in the time frame scale. Nondynamic + ends superimpose on each other in all 90 frames due to their static nature and represent as white due to superimposition, whereas dynamic + ends do not superimpose in all the 90 frames due to their dynamic behavior and thus appear as a rainbow color. Thus, higher dynamics of tubulin result in a wider range of colors in these representative superimposed images. ( B ) Quantitative representation of the microtubule (MT) growth speed in control and CKAP5 -silenced group. The live-cell kinetic data obtained from a spinning disk microscope was subjected to Utrack analysis using MATLAB software. ** P = 0.0097. ( C ) Quantitative representation of the MT growth lifetime in control- and CKAP5 -silenced group as measured by MATLAB analysis. ( D ) Quantitative representation of the MT growth length in control and CKAP5 -silenced group as measured by MATLAB analysis. For all the graphical analysis in (B) to (D), the data are represented as mean ± SEM from 10 mitotic events and statistically analyzed by an unpaired t test.

    Article Snippet: EB3 dynamics was quantified by using the utrack MATLAB analysis method as mentioned in the protocol.

    Techniques: Control, Transfection, Plasmid Preparation, Live Cell Imaging, Microscopy, Software